[Clinical characteristics of hypertensive encephalopathy in the perimenopausal period].

OBJECTIVES: To study correlations between symptoms of hypertensive encephalopathy (HE), structural MRI changes of the brain and affective disorders in the perimenopausal period. MATERIALS AND METHODS: A study included 150 women who have been through natural menopause (group 1) and 100 women in the premenopausal (group 2). Somatic and neurological examination, MRI and psychometric scales for anxiety and depression were used. RESULTS AND CONCLUSIONS: Women with hypertensive encephalopathy in the postmenopausal period had signs of persistent psycho-emotional disorders (higher anxiety, depression of different severity) combined with structural changes in the brain, with their severity increasing with the progression of HE.

[Morphology and medical correction of reparative regeneration processes in corneal injury].

Analysis of current literature on morphology of wound processes in the cornea during up-to-date techniques effecting keratogenesis is performed.

[The psychic state of women suffering infertility in the old reproductive age].

A total 60 infertile women aged 36-42 years treated by ART were examined to evaluate their psychic condition using CES-D and Spielberg-Khanin scales, SF-36 questionnaire. 15 (25%) patients were found (CES-D) to have mild depressive disorder; it was more pronounced in one woman. More than half of the patients exhibited enhanced anxiety, with 32 (53.3%) and 19 (31.6%) having generalized and strong anxiety respectively. Estimation of the quality of life revealed difficulties in maintaining social contacts up to complete isolation, low self-assessment of psychic health and prospects for recovery. It is concluded that comprehensive diagnostics and combined treatment are needed to manage infertile women of old reproductive age with the use of both traditional and psychocorrective methods.

[Melatonin secretion in women of advanced reproductive age].

The patient's age is a key factor determining success of in vitro fertilization. The ovarian reserve and oocyte quality are known to decrease with age. Much attention has been given recently to the role of epiphysis and its hormone, melatonin, in synchronization of daily and seasonal biorhythms in anti-stress protection and neuroregulation of reproductive processes. The aim of our work was to study melatonin levels in infertile women of reproductive age. We also measured sex hormones, anti-Mullerian hormone, FSH, and LH in blood and melatonin sulfate in urine at 8 points (RIA). Women of advanced reproductive age showed markedly reduced melatonin secretion due to functional disorders in the hypothalamic-pituitary-gonadal axis. Results of the study suggest the necessity of prescription of exogenous melatonin to the patients included in assisted reproduction programs for the improvement of their efficacy.

[Thrombocytic and coagulation hemostasis in pregnant women living in the Ob-Irtysh river basin with chronic opisthorchiasis].

Opisthorchiasis that is widely spreading in Russia and CIS countries was and remains a serious social, medical, and scientific problem. Local or imported cases of opisthorchiasis have been notified in 87.6% of the administrative territories of Russia. The world's largest focus of opisthorchiasis is the Ob-Irtysh river basin with natural preconditions for its high infection in fish. The main clinical manifestations of Opisthorchis infestation are associated with liver damage caused by the obligate presence of Opisthorchis in the bile capillaries with evolving cholangitis, cholangiocholecystitis, and cholangiopancreatitis. Opisthorchis complicates the course of many diseases, affecting gestation, labor, and postpartum. The state of thrombocytic and coagulation hemostasis was analyzed in different period of pregnancy and postpartum (days 2-4), by using the course of pregnancy in the inhabitants of the hyperendemic focus of Opisthorchis infestation (the Ob-Irtysh basin) as an example. In the newcomers, pregnancy develops in the presence of platelet hyperaggregation and accelerated continuous blood clotting. In pregnant women who belong to native little peoples (Khanty, Mansi), opisthorchiasis induces no significant changes in the hemostatic potential.

[Analysis of nuclear protein complexes comprising alpha-actinin-4 by 2D-electrophoresis and mass-spectrometry].

Actin-binding protein alpha-actinin-4 is a member of spectrin super family. It is located in the cytoplasm and in the nucleus. However, nuclear functions of alpha-actinin-4 are still not clear. In this study, we analyzed composition of nuclear protein complexes associated with alpha-actinin-4 in A431 cells. Using 2D electrophoresis, we have determined that about 50 different proteins may be associated with nuclear alpha-actinin-4. Using mass-spectrometry, we analyzed major proteins of these complexes. beta-Actin, alpha- and beta-tubulins, ribonucleoprotein A2/B1, which regulates splicing and is associated with beta-actin, peroxiredoxin-1, which is involved in oxidative stress, and glycolytic enzyme D-3-phosphoglycerate dehydrogenase were identified by MALDI-TOF. Detection of these proteins in nuclear complexes with alpha-actinin-4 may suggest that alpha-actinin-4 is involved in transcription and splicing. Presence of beta-actin in the investigated complexes was confirmed by tandem mass-spectrometry (MALDI-TOF-TOF). Immunoprecipitation of nuclear proteins with antibodies against alpha-tubulin confirmed association of alpha-actinin-4 with alpha-tubulin in the protein complex. Nuclear alpha-actinin-4 constitutes of 105 KDa fullsize isoform and two truncated isoforms of 65 and 75 kDa, whereas only the truncated isoform have been found in nuclear complexes with alpha-tubulin. These data suggest that alpha-actinin-4 is associated with a number of different nuclear protein complexes which may carry out different functions in the cell nucleus.

[Impact of Opisthorchis invasion on lipid peroxidation and hemostasis during pregnancy].

Blood cell lipid peroxidation processes and the state of thrombocytic and coagulative hemostasis were studied in 214 pregnant women and parturients with Opisthorchis invasion amongst the indigenous population and newcomers of the Khanty-Mansi Autonomic District-Yugra. Chronic opisthorchiasis was found to enhance lipid peroxidation in the blood cells, to accelerate the activation of thrombocytic and coagulative hemostasis during gestation, at labor, and in the postpartum period. Opisthorchis invasion variously affected the state of lipid peroxidation and hemostasis in the examined groups. In the indigenous women, the number of activated forms of platelets increased, without any obvious change in their functional potential, which was unaccompanied by changes in the parameters of coagulative hemostasis and lipid peroxidation. Increased activation of platelets, their hyperaggregability, and subsequent depletion of their functional potential were revealed in the newcomers. This was attended by hypercoagulation with signs of acceleration of continuous blood coagulation, by enhanced lipid peroxidation and reduced antioxidative potential in the blood cells.

[Control of industrial waste consumption residues: ecological and hygienic aspects].

The problem in the provision of safe handling of industrial waste and consumption residues is relatively current. According with the United Nations Organization's data, 25 to 33% of the world's notified diseases are directly associated with the low quality of the human environment. Up to now, a list of chemicals encountered in the waste and residues is unavailable in Russia and foreign countries. By keeping in mind the ubiquitous spread of industrial waste and consumption resides due to human vital activity, their huge formations and their very wide diversity in composition, type, and pattern of a possible dangerous effect, it is important to consider the problem associated with waste handling, by evaluating their environmental and hygienic hazard.

[The role of intravascular autorossette-formation in the onset of anemia in patients with chronic cardiac failure].

AIM: To study intensity of intravascular autorossette-formation (ARF) and its role in development of anemia in patients with chronic heart failure (CHF). MATERIAL AND METHODS: Blood smears (Romanovsky-Gimze stain) were obtained from 210 patients with CHF (functional class I-III) treated as indicated. Total number of autorossettes (AR) and AR with red cell lysis in them were calculated in the blood smears. RESULTS: The number of endogenic AR increases with higher functional class of CHF, duration of the disease, number of drugs. With increasing number of AR and AR with exocytic lysis, number of red cells and blood hemoglobin concentration lower. CONCLUSION: It is suggested that phenomenon of intravascular autorossette-formation is involved in pathogenesis of anemia in CHF patients and a cause of anemia may be much more complicated that it was thought initially.

[Nitric oxide in the lymphatic microvessel regulation]. / Uchastie oksida azota v reguliatsii funktsii limfaticheskikh mikrososudov.

Topical application of sodium nitroprusside on rat mesentery has a marked influence on lymph microvessels function. The drug causes a dilation of majority of lymphangions and decrease of the pacemaker activity of the vessel wall structures and valves. These changes do not lead to lymph stasis, and lymph flow velocity does not reduce. The non-selective inhibitor of NO synthase (N-nitro-L-arginine) intensifies vasomotions of lymph microvessels, modulates phasic contractile activity and increases lymph flow velocity. There is a time dependent dynamic of changes in action of N-nitro-L-arginine. During inhibition of endogenous NO synthesis the responses of lymph microvessels on sodium nitroprusside application are modified: the constriction of majority lymphangions and activation of valve work are observed.

Changes in lymph microcirculation during pathological stress.

Stress caused by immobilization and acoustic stimulation produces considerable changes in lymph microcirculation. These changes manifested in dilation of microvessels, enhanced phasic contractile activity and its abnormal pattern, accelerated lymph flow, and recruitment of new lymphangions in drainage functions.

[Functional organization of lymphatic microvessels of the rat mesentery]. / Funltsional'naia organizatsiia limfaticheskikh mikrososudov bryzheiki krysy.

Lymph flow as measured with biomicroscopic and speckle-interferometrical techniques was very unstable and its rate did not depend on lymphangion diameter in the rat mesentery. A correlation was found between amount of cells in the lymph flow and other indices of lymphangion functions. All microvessels with phasic contractions and/or working valve were revealed as having a lymph flow. The phasic activity and the valve work correlated with each other. The higher the amplitude and frequency of the phasic activity and the longer the contraction cycle, the higher was the rate of the valve work.